Literature summary extracted from
Noguchi, S.
Isomerization mechanism of aspartate to isoaspartate implied by structures of Ustilago sphaerogena ribonuclease U2 complexed with adenosine 3'-monophosphate (2010), Acta Crystallogr. Sect. D, 66, 843-849.
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
4.6.1.20 |
ribonuclease U2 complexed with adenosine 3'-monophosphate, hanging drop vapour diffusion method, 0.005 ml of protein solution containing 20 mg/ml RNase U2A and 8 mM adenosine 3'-monophosphate, pH 4.5, is mixed with 0.005 ml of reservoir solution containing 12.5% w/w PEG 8000, 200 mM calcium acetate, 100 mM sodium cacodylate, pH 3.75, 12.5% w/w PEG 8000, equilibration against 0.4 ml reservoir solution, 20°C, 3 days, X-ray diffraction structure determination and analysis at 0.96-0.99 A resolution |
Ustilago sphaerogena |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
4.6.1.20 |
Ustilago sphaerogena |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
4.6.1.20 |
native RNase U2A by anion-exchange and 5'-adenylate aminohexyl affinity chromatography |
Ustilago sphaerogena |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
4.6.1.20 |
RNase U2A |
- |
Ustilago sphaerogena |
General Information
EC Number |
General Information |
Comment |
Organism |
---|
4.6.1.20 |
additional information |
mechanism of isoaspartate formation within the Asp45-Glu46 sequence of ribonuclease U2, overview. In the succinimide-formation reaction water 219 receives a proton from the N atom of Glu46 as a general base and waters 190 and 220 stabilize the tetrahedral intermediate, and in the succinimide-hydrolysis reaction water 219 provides a proton for the N atom of Glu46 as a general acid |
Ustilago sphaerogena |